Quick Assembly Cloning Kit
Catalog No.: 2901-020
Size: 20 rxns
Description
Quick Assembly Cloning Kit is a kind of seamless assembly method, digest, ligate or end repair are not required. This method has been used to assemble different sizes of DNA fragments with varied overlaps (15~30 bp). And it offers a easy, fast and efficient DNA cloning technique.
This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. It can accomplish single fragment assembly in as little as 5 minutes with a high positive rate exceeding 95%. The mix contains enhancers that improve the cloning efficiency, while the reaction system was optimized, so no PCR clean-up step is required.
This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. It can accomplish single fragment assembly in as little as 5 minutes with a high positive rate exceeding 95%. The mix contains enhancers that improve the cloning efficiency, while the reaction system was optimized, so no PCR clean-up step is required.
Components
| Component |
Amount |
| Quick Assembly Cloning Mix | 100 μl |
| pUC19 Control Plasmid, Linearized (Ampr, 40 ng/μl) | 2 μl |
| 500 bp Control Fragment (20 ng/μl) | 2 μl |
Storage Condition
-20℃
