Flash HindIII
Catalog No.: 2512-500
| Size： | 500 rxns |
| Cut site： | AAGCTT |
| 5'...A ↓ A G C T T...3' 3'...T T C G A ↑ A...5' |
Description
Flash enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All Flash enzymes show superior activity in the universal Flash and Flash Color Buffer, and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. Flash enzymes have passed multiple strict quality controls, and can be used to digest plasmid, genomic and viral DNA as well as PCR products.
Flash Color Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the Flash Color Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye
migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.
Flash Color Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the Flash Color Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye
migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.
Components
| Component | Amount |
| Flash HindIII (20 U/μl) | 500 μl (10000 U) |
| 10× Flash Buffer | 3×1 ml |
| 10× Flash Color Buffer | 3×1 ml |
Features and Usage
The reaction can be completed within 15 minutes
Recommended Reaction Conditions
1× Flash Buffer；
Incubate at 37℃；
Refer to "Protocol for Fast DNA Digestion" for reaction setup.
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Methylation sensitivity
Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the EcoBI methylase.
Storage Condition
-20℃