Alkaline Phosphatase (Flash)

 

    Alkaline Phosphatase (Flash) is an enzyme that catalyzes the hydrolysis of the 5' and 3' phosphate groups of DNA, RNA, and nucleotides. It can also remove protein phosphate groups but does not hydrolyze phosphodiester and phosphotriester bonds. This enzyme can dephosphorylate the ends of all types of DNA in 10 minutes at 37℃ . It has 100% activity in Flash buffer and is inactivated by incubation at 80 ℃ for 20 minutes. When used together with Flash restriction endonucleases, it simplifies the "digestion-dephosphorylation" reaction in one tube.