RNAGuard RNase Inhibitor

 

    RNAGuard RNase Inhibitor is a 50 kDa recombinant protein of murine origin. The inhibitor specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).
   RNAGuard RNase Inhibitor does not contain the pair of cysteines identified in the human version that is very sensitive to oxidation, which causes inactivation of the inhibitor. As a result, RNAGuard RNase Inhibitor has significantly improved resistance to oxidation compared to the human/porcine RNase inhibitors, and is stable at low DTT concentrations (less than 1 mM). This makes it ideal for reactions where high concentration DTT is adverse to the reaction (eg. Real-time RT-PCR).
Applications:
1. Inhibition of common eukaryotic RNases.
2. cDNA synthesis and RT-PCR.
3. In vitro transcription and translation.
4. Enzyme-catalyzed RNA labeling reactions.
5. Other applications where the integrity of RNA is important.