Nt.BspQI
Catalog No.: 2564-200
| Size： | 200 rxns |
| Cut site： | 5'...G C T C T T C N↓...3' 3'...C G A G A A G N ...5' |
Description
Nt.BspQI is a nicking endonuclease that cuts only one strand of the dsDNA substrate; it introduces a nick into the dsDNA substrate without cleaving the dsDNA.
Components
| Component | Amount |
| Nt.BspQI (10 U/μl) | 200 μl |
| 10× Reaction Buffer | 2×1 ml |
Features and Usage
Recommended Reaction Conditions
1× Reaction Buffer;
Incubate at 50℃ ;
Refer to "Protocol for DNA Digestion" for reaction setup.
This product has 75% activity when performing enzymatic digestion reactions at 37℃ .
Incubate at 50℃ ;
Refer to "Protocol for DNA Digestion" for reaction setup.
This product has 75% activity when performing enzymatic digestion reactions at 37℃ .
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Storage Condition
-20