Nb.BsrDI
Catalog No.: 2566-200
| Size： | 200 rxns |
| Cut site： | 5'...G C A A T G N N...3' 3'...C G T T A C↑N N ...5' |
Description
Nb.BsrDI is a nicking endonuclease that only cuts one strand of the dsDNA substrate, resulting in nicks on the dsDNA without complete cleavage.
Components
| Component | Size |
| Nb.BsrDI (10 U/μl) | 200 μl |
| 10× Flash Buffer | 2×1 ml |
| 10× Flash Color Buffer | 2×1 ml |
Features and Usage
Recommended Reaction Conditions
1× Flash Buffer；
Incubate at 65℃ ;
Refer to "Protocol for DNA Digestion" for reaction setup.
This product has 50% activity when performing enzymatic digestion reactions at 37℃ .
Incubate at 65℃ ;
Refer to "Protocol for DNA Digestion" for reaction setup.
This product has 50% activity when performing enzymatic digestion reactions at 37℃ .
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Storage Condition
-20