Nt.BstNBI
Catalog No.: 2570-200
| Size： | 200 rxns |
| Cut site： | 5'...G A G T C N N N N N↓...3' 3'...C T C A G N N N N N ...5' |
Description
Nt.BstNBI is a nicking endonuclease that only cuts one strand of the dsDNA substrate, resulting in nicks on the dsDNA without complete cleavage.
Components
| Component | Size |
| Nt.BstNBI (10 U/μl) | 200 μl |
| 10× Reaction Buffer | 2×1 ml |
Features and Usage
Recommended Reaction Conditions
1× Reaction Buffer ;
Incubate at 55℃ ;
Refer to "Protocol for DNA Digestion" for reaction setup.
This product has 50% activity when performing enzymatic digestion reactions at 37℃ .
Incubate at 55℃ ;
Refer to "Protocol for DNA Digestion" for reaction setup.
This product has 50% activity when performing enzymatic digestion reactions at 37℃ .
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Storage Condition
-20