2 X Robust PCR Mix (Dye)

 

   2X Robust PCR Mix (Dye) is a ready-to-use PCR mix with the concentration of 2X. lt offers convenience and reduces the risk of contamination. PCR products synthesized with this mix have an overhang of A bases at the 3' end, enabling direct T/A cloning after purification.
   The Taq DNA polymerase in this mix is a mutated variant, which is able to amplify DNA fragments up to 5 kb efficiently. It exhibits excellent tolerance to inhibitors and has a three-fold faster amplification rate compared to wild type Taq DNA polymerase, significantly reducing the time required for PCR extension. Unlike fusion proteinbased fastTaq polymerases, this mutated Taq behaves more similarly to WT-Taq, minimizing issues such as band smearing, trailing, or alteration of fragment sizes.
    The PCR mix contains two dyes, after reaction the sample could be loaded directly for gel electrophoresis. The dyes do not affect PCR amplification efficiency. However, for experiments requiring optical analysis such as absorbance or fluorescence of PCR products, it is recommended to purify the PCR product before analysis.