T4 gp32 ssDNA binding protein

 

    T4 gp32 ssDNA binding protein is a single-strand DNA binding protein encoded by the T4 bacteriophage gene 32. It has a molecular weight of 35 kDa and is an essential component for DNA replication and repair in the T4 bacteriophage. It cooperatively binds to and stablizes transiently formed regions of ssDNA and plays an important structural role during T4 phage replication. It also has been used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structures. T4 gp32 ssDNA binding protein can facilitate restriction enzyme digestion reactions, improve efficiency of reverse transcription in RT-PCR, enhance DNA polymerase activity, and can be used in recombinase polymerase amplification (RPA) reactions.