TBE

 

     TBE appears as white instant granules. Each bag makes 1 L of 1× TBE solution conveniently with a simple procedure. TBE is composed of Tris, boric acid and EDTA. The final concentration of the 1× solution is 89 mM Tris, 89 mM boric acid and 2 mM EDTA.
     TBE buffer is a nucleic acid electrophoresis buffer commonly used in biology labs. It is mainly used in agarose gel electrophoresis of DNA. It has a good buffering capacity and shows good results when separating DNA fragments smaller than 1 kb. It is also suitable for long-time electrophoresis. TBE buffer has a high electroosmosis effect with agarose gel and forms tetrahydroxy borate complexes with the agarose through nonconvalent binding, which impairs the DNA recovery rate. Thus, TBE buffer is not recommended for recovering DNA from agarose gel after electrophoresis.