T5 Exonuclease
Catalog No.: 2615-100
Size: 100 rxns
Description
T5 Exonuclease is a nucleic acid exonuclease that digests DNA from the 5' end in the 5'→3' direction for both double-stranded and single-stranded DNA. It is capable of digesting DNA at nicks or gaps in linear or circular double-stranded DNA,however, it cannot digest supercoiled DNA. In addition, when the Mg2+ concentration in the solution is below 1 mM, the activity of T5 Exonuclease towards single-stranded DNA is inhibited. Consequently, T5 Exonuclease is particularly suitable for degrading linearized and nicked plasmids, removing non-circular DNA from ligation products, seamless cloning (Gibson Assembly), and other applications.
Components
| Component |
Amount |
| T5 Exonuclease (10 U/μl) | 100 μl |
| 10× Reaction Buffer | 1 ml |
Storage Condition
-20℃