FaithScriptor G4th RT Supermix
Catalog No.: 2209-100
Size: 100 rxns
The FaithScriptor G4th Reverse Transcriptase in the premix is a newly developed 4th-generation reverse transcriptase. It features faster reaction speed, completing the reverse transcription process within 5 minutes; stronger thermostability with an optimal reaction temperature of 55 °C; the cDNA product can reach up to 20 kb in length. Additionally, it exhibits tolerance to common inhibitors such as ethanol, isopropanol, humic acid, lithium chloride, and guanidine hydrochloride.
dsDNase can specifically digest double-stranded DNA even in the presence of primers and probes, without degrading single-stranded DNA or RNA. It exhibits thermal sensitivity, enabling rapid irreversible inactivation at elevated temperatures to eliminate genomic contamination. This prevents dsDNase from damaging DNA within DNA-RNA hybrid strands during reverse transcription.
Description
FaithScriptor G4th RT Supermix (with dsDNase) is a simplified system specially designed for single-stranded cDNA synthesis. It contains all components required for the reaction, requiring only the addition of RNA template and water.
The FaithScriptor G4th Reverse Transcriptase in the premix is a newly developed 4th-generation reverse transcriptase. It features faster reaction speed, completing the reverse transcription process within 5 minutes; stronger thermostability with an optimal reaction temperature of 55 °C; the cDNA product can reach up to 20 kb in length. Additionally, it exhibits tolerance to common inhibitors such as ethanol, isopropanol, humic acid, lithium chloride, and guanidine hydrochloride.
dsDNase can specifically digest double-stranded DNA even in the presence of primers and probes, without degrading single-stranded DNA or RNA. It exhibits thermal sensitivity, enabling rapid irreversible inactivation at elevated temperatures to eliminate genomic contamination. This prevents dsDNase from damaging DNA within DNA-RNA hybrid strands during reverse transcription.
Components
| Component |
Size |
| FaithScriptor G4th RT Supermix | 400 μl |
| dsDNase | 2 x 50 μl |
| 10× dsDNase Buffer | 200 μl |
| Nuclease-Free Water | 2 x 1 ml |
Storage Condition
Store at -20℃