Tris-Glycine
Catalog No.: 2800-005
Size: 5 bags
Note：pH is 8.3±0.2@25℃ when made to 1× solution.
Description
Tris- Glycine(hereinafter referred to as TG) appears as white to pale yellow instant granules . Each bag makes 1 L of 1 × TG , which is easy to operate and easy to use. The main components of TG are Tris and Glycine. The concentration of Tris in 1 × TG is 25 mM Tris and the concentration of glycine in 1 × TG is 192 mM.
① TG buffer is commonly used for rapid western blotting after being configured in 20% ethanol for 1×, mainly for wet turning.
② For the preparation of non-denaturing protein polyacrylamide gel electrophoresis buffer.
① TG buffer is commonly used for rapid western blotting after being configured in 20% ethanol for 1×, mainly for wet turning.
② For the preparation of non-denaturing protein polyacrylamide gel electrophoresis buffer.
Components
| Component | Size |
| Tris-Glycine | 5 bags |
Protocol
1. Put magnetic stirring beads and ~600 ml distilled water into a beaker.
2. While stirring, slowly pour the whole contents from 1 bag of TG into the beaker; wait until everything is dissolved.
3. Add distilled water to bring the volume to 1 L and 1 × solution is made.
2. While stirring, slowly pour the whole contents from 1 bag of TG into the beaker; wait until everything is dissolved.
3. Add distilled water to bring the volume to 1 L and 1 × solution is made.
Storage Condition
Store at room temperature for three years