PNGase F, His-tag (500 U/ul)

 

PNGase F (Peptide N-Glycosidase F) is a high effective amidase derived from Elizabethkingia miricola . It cleaves asparagine-linked high mannose as well as hybrid and complex oligosaccharides from glycoproteins. The cleavage site of PNGase F is the amide bond between the N-acetylglucosamine (GlcNAc) inside the protein and the asparagine residue, while converting the asparagine residue in the digested protein to aspartic acid.

This product is expressed through recombinant expression in E.coli , and it contains a His tag and 50% glycerol. It is commonly used for deglycosylation of antibodies and related proteins.