TBS
Catalog No.: 2001-005
Size: 5 bags
Note：pH is 7.4±0.15@25℃ when made to 1× solution.
Description
TBS appears as white to off-white instant granules. Each bag makes 1 L of 1× TBS solution conveniently with a simple procedure. TBS is composed of NaCl, KCl and Tris. 1× TBS contains 137 mM NaCl, 2.7 mM KCl and 25 mM Tris.
TBS buffer is a widely used isotonic buffer in biology labs. It is used in immunohistochemistry, in situ hybridizations, enzyme-linked immunoassays and western blot. It is ideal for substrate washing, antibody dilution and blocking solution preparation.
TBS buffer is a widely used isotonic buffer in biology labs. It is used in immunohistochemistry, in situ hybridizations, enzyme-linked immunoassays and western blot. It is ideal for substrate washing, antibody dilution and blocking solution preparation.
Components
| Component | 2001-005 |
| TBS | 5 bags |
Protocol
1. Put magnetic stirring beads and ~600 ml distilled water into a beaker.
2. While stirring, slowly pour the whole contents from 1 bag of TBS into the beaker; wait until everything is dissolved.
3. Add distilled water to bring the volume to 1 L and 1× solution is made.
2. While stirring, slowly pour the whole contents from 1 bag of TBS into the beaker; wait until everything is dissolved.
3. Add distilled water to bring the volume to 1 L and 1× solution is made.
Storage Condition
Store at room temperature for three years