DNase I, RNase-free
Catalog No.: 2608-01K
Size: 1000 U
Description
DNase I, RNase-free, is an endodeoxyribonuclease that catalyzes to the same extent the degradation of both single- and double-stranded DNA randomly, and produces 5'-P terminal oligonucleotides.
The activity of DNase I is dependent on Ca2+, and can be activated by bivalent metal ions Mg2+, Mn2+, etc. In the presence of Mg2+, the enzyme can randomly recognize and cut any site on any strand of double-stranded DNA. In the presence of Mn2+, nearly identical sites on the two strands of DNA can be recognized and cut, resulting in flat ends or sticky end DNA fragments with 1 to 2 nucleotides overhang.
The activity of DNase I is dependent on Ca2+, and can be activated by bivalent metal ions Mg2+, Mn2+, etc. In the presence of Mg2+, the enzyme can randomly recognize and cut any site on any strand of double-stranded DNA. In the presence of Mn2+, nearly identical sites on the two strands of DNA can be recognized and cut, resulting in flat ends or sticky end DNA fragments with 1 to 2 nucleotides overhang.
Components
| Component |
Amount |
| DNase I, RNase-free(1 U/μl) |
1000 U |
| 10×DNase I Buffer |
1.25 ml |
Storage Condition
-20℃